特徴
- Flamma Fluors 552 Maleimide is a thiol reactive bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 550/564 nm, similar to that of Alexa 555, DyLight 549 ATTO 550 and Cy 3. Flamma 552 might be excited using 543, 546 or 555 nm laser line and displays excellent optical property. Flamma 552 maleimide can be conjugated to low-abundance of biomolecules with great sensitivity allowing sensitive detection. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. BioActs offers Flamma 552 maleimide for labeling of thiols on antibodies, peptides, proteins, ligands and amplification substrates optimized for cellular labeling and detection.
仕様
- サイズ:1MG
- Fluorophore: Flamma Fluors 552
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 550/564
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550, CF555
- Extinction coefficient: ≥ 136,000 cm-1M-1
- CF280: 0.07
- Appearance: Red Solid
- Molecular weight: 793.3 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
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- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL:06-6447-8641
- FAX:06-6447-8642
- E-mail:[email protected]
商品のバリエーション (サイズ違い・スペック違い・オプション品など)
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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83-6100-25
Flamma 552 Maleimide 1MG
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83-6100-27
Flamma 552 Maleimide 25MG
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83-6100-26
Flamma 552 Maleimide 5MG
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