特徴
- Flamma Fluors 496 Maleimide is a thiol reactive bright green dye induced from fluorescein structure and used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 496/520 nm, similar to that of Alexa 488 and FAM. Flamma 496 might be excited using 488 nm laser line and displays excellent optical property. Flamma 496 maleimide can be conjugated to low-abundance of biomolecules with great sensitivity allowing sensitive detection. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. BioActs offers Flamma 496 maleimide for labeling of thiols on antibodies, peptides, proteins, ligands and amplification substrates optimized for cellular labeling and detection.
仕様
- サイズ:1MG
- Fluorophore: Flamma Fluors 496
- Reactive group: Maleimide
- Excitation/Emission Max.(nm): 496/520
- Spectrally similar dyes: Alexa 488, FAM
- Extinction coefficient: ≥ 64,000 cm-1M-1
- CF280: 0.12
- Appearance: Yellow to Orange Solid
- Molecular weight: 536.44 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
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- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL:06-6447-8641
- FAX:06-6447-8642
- E-mail:[email protected]
商品のバリエーション (サイズ違い・スペック違い・オプション品など)
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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83-6100-22
Flamma 496 Maleimide 1MG
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83-6100-23
Flamma 496 Maleimide 5MG
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83-6100-24
Flamma 496 Maleimide 25MG
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