RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes.
Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors.
Pol II can coordinate 10 to 14 subunits.
This complex interacts with the promoter regions of genes and a variety of elements and transcription factors.
The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Applications:Suitable for use in Western Blot and ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 0.05-1ug/ml detected RNA polymerase II in HeLa nuclear extracts
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
抗体種類：Pab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes phosphorylated and unphosphorylated forms RNA polymerase Species Crossreactivity: human, rat, mouse and Saccharomyces cerevisiae.
Clone # USB：CTD4H8
Isotype：IgG1
Calc Applications Abbrev：ChIP WB
Calc Crossreactivity：Hu Mo Rt Ye
Immunogen：Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
