83-6076-24　MYLK (DKFZp686I10125, FLJ12216, KRP, mLCK, mLCK1, mLCK108, mLCK210, MSTP083, MYLK1, smmLCK) 50ug　470077

特徴

• This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme.
• This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity.
• This gene encodes both smooth muscle and nonmuscle isoforms.
• In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments.
• A pseudogene is located on the p arm of chromosome 3.
• Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.
• Additional variants have been identified but lack full length transcripts.
• [provided by RefSeq]
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLA KKGTSKTPVPEKVPPPKPATPDFRSVLGGKKKLPAEN GSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAKPA ETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKK DVKNDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQ DVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFI ILSQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECS CQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESD ATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVEL FGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGSK LTILAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPD PPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEI WDSANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAI NVYGTSEPSQESELTTVGEKPEEPKDEVEVSDDDEKE PEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLV EKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPK LVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTE RECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTG TRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPI GYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSA TWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCL QHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTG NAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAEKLE SEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSA ARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDED GNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVET MEEGEGEGEEEEE
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.