Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER).
Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER.
Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.