83-6002-20 HIF3A (Hypoxia-inducible Factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS Protein MOP7, Class E Basic Helix-loop-helix Protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS Domain Protein, IPAS, Member of PAS Protein 7, PAS Domain-containing Protein 7, BHLHE17, MOP7, PASD7) 50ug 127855
83-6002-20 HIF3A (Hypoxia-inducible Factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS Protein MOP7, Class E Basic Helix-loop-helix Protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS Domain Protein, IPAS, Member of PAS Protein 7, PAS Domain-containing Protein 7, BHLHE17, MOP7, PASD7) 50ug 127855
Suppresses hypoxia-inducible expression of HIF1A and EPAS1.
Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters.
The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE.
Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription.
Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription.
Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia.
Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity.
Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis.
Functions as an inhibitor of angiogenesis in the cornea.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
AA Sequence:MALGLQRARSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGSPDSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHALDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISRVEETGVVLSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALDLEMLAPYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDPSASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
抗体種類：Pab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human HIF3A.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Full length human HIF3A, aa1-669 (AAH80551.1).
