83-5995-59　DCIR (C-type Lectin Domain Family 4, Member A, C-type Lectin Superfamily Member 6, Dendritic Cell Immunoreceptor, Clec4a2, Clecsf6, Dcir, CLEC4A) 50ug　125663

特徴

• A member of the calcium-dependent (C-type) lectin receptor (CLR) family, DCIR (for dendritic cell (DC) immunoreceptor), is a type II membrane glycoprotein of 237aa with a single carbohydrate recognition domain (CRD), closest in homology to those of the macrophage lectin and hepatic asialoglycoprotein receptors.
• The intracellular domain of DCIR contains a consensus immunoreceptor tyrosine-based inhibitory motif (ITIM).
• A mouse cDNA, encoding a homologous protein has been identified.
• Northern blot analysis shows that DCIR mRNA is predominantly transcribed in hematopoietic tissues.
• DCIR displays a typical lectin CRD and is expressed on DCs, monocytes, macrophages, B lymphocytes, and granulocytes, but not detected on NK and T cells.
• DCIR is strongly expressed by DCs derived from blood monocytes cultured with GM-CSF and IL-4.
• DCIR is preferentially expressed by monocyte-related rather than Langerhans cell related DCs obtained from CD34+ progenitor cells.
• Finally, DCIR expression is down-regulated by signals inducing DC maturation such as CD40 ligand, LPS, or TNF-alpha.
• Thus, DCIR is differentially expressed on DCs depending on their origin and stage of maturation:activation.
• DCIR represents a novel surface molecule expressed by antigen-presenting cells, and of potential importance in regulation of DC function.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MTSEITYAEVRFKNEFKSSGINTASSAASKERTAPHKSNTGFPKLLCASLLIFFLLLAISFFIAFVIFFQKYSQLLEKKTTKELVHTTLECVKKNMPVEETAWSCCPKNWKSFSSNCYFISTESASWQDSEKDCARMEAHLLVINTQEEQDFIFQNLQEESAYFVGLSDPEGQRHWQWVDQTPYNESSTFWHPREPSDPNERCVVLNFRKSPKRWGWNDVNCLGPQRSVCEMMKIHL
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.