The annexin superfamily consists of 13 calcium or calcium and phospholipid binding proteins with high biological and structural homology.
Annexin-1 (ANXA1) is the frst characterized member of the annexin family of proteins able to bind to cellular membranes in a calcium-dependent manner which promotes membrane fusion and is involved in endocytosis.
Annexin A1 has anti-infammatory properties and inhibits phospholipase A2 activity.
Annexin A1 can accumulate on internalized vesicles after EGF-stimulated endocytosis,suggesting that it may be required for a late stage in inward vesiculation.Phosphorylation by PKC, EGFR and Chak1 results in inhibition of annexin A1 function.
Annexin A1 has also been identifed as one of the 'eat-me' signals on apoptotic cells to be recognised and ingested by phagocytes.
Annexin A1, as an endogenous anti-infammatory mediator, has roles in many diverse cellular functions, such as membrane aggregation, infammation, phagocytosis,proliferation, apoptosis, and tumorigenesis and cancer development.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 10ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 3ug/mlOptimal dilutions to be determined by the researcher.
AA Sequence:MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
抗体種類：Pab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human ANXA1.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Full length human ANXA1, aa1-346 (NP_000691.1).
