Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested
Recommended Dilutions:ELISA: 1:14,000 to 1:60,000, 30 minutes at RT
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4℃ for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20℃.
Reconstituted product is stable for 12 months at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
抗体種類:Pab
宿主:mouse
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/mL BSA, 0.01% sodium azide. Reconstitute with 1mL sterile, distilled, deionized water or sterile 40-50% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per mouse IgG molecule.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human, Goat and Mouse Serum Proteins.