83-5985-92　Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor) 1mg　L1670-10

特徴

• Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis.
• Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R.
• Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
• Recombinant mouse leptin is a 16.2kD protein containing 147 amino acid residues.
• Biological Activity:Determined by an-in vivo-assay using the ob/ob mouse obesity model and NZO.
• Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5μg leptin/gm of body weight for 7 days.
• Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.
• Amino Acid Sequence:MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20℃.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.