83-5985-86　Interleukin 1 alpha, Recombinant, Mouse (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) 10ug　I7663-01

特徴

• IL-1a is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes and other epithelial cells.
• Both IL-1a and IL-1b bind to the same receptor and have similar, if not identical, biological properties.
• These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells.
• However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine.
• Recombinant mouse IL-1a is a 17.9kD protein containing 156 amino acid residues.
• Amino Acid Sequence:SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
• Biological Activity:The ED50 as determined by the dose-dependent stimulation of murine D10S cells is ≤0.002ng/ml, corresponding to a specific activity of ≥5x10e8 units/mg.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20℃.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.