83-5985-77　Ciliary Neurotropic Factor, Recombinant, Rat (CNTF) 5ug　C5073

特徴

• Ciliary Neutrophic Factor (CNTF) is a potent neural factor originally characterized as a survival factor for chick ciliary neurons in vitro.
• CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes.
• CNTF is highly conserved across species and exhibits cross-species bioactivity.
• Recombinant rat CNTF is a 22.7kD protein containing 199aa residues, lacking a hydrophobic N-terminal signal for secretion.
• Biological Activity:Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 25-35ng/ml.
• Amino Acid Sequence:AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20℃.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.