Eosinophil peroxidase (UniProt: P11678; also known as EC:1.11.1.7, EPO, EPX) is encoded by the EPX (also known as EPER, EPO, EPP) gene (Gene ID: 8288) in human.
Eosinophil peroxidase is an enzyme found in cytoplasmic granules of eosinophils.
It mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils.
It is synthesized with a signal peptide (aa1-17) and a propeptide (aa18-139), which are subsequently cleaved off to produce the mature form that can be cleaved into a light chain (aa140-250) and a heavy chain (aa251-715).
These chains can assemble to for a tetrameric structure.
It is a heme-containing enzyme that physically associates with fibrous tissue and cancer tissue in various organs.
It contains well-conserved profibrogenic capacity to stimulate the migration of fibroblastic cells and promote their ability to secrete collagenous proteins to generate a functional extracellular matrix (ECM) both in vitro and in vivo.
It plays a role in regulating the recruitment of fibroblast and the biosynthesis of collagen ECM at sites of normal tissue repair and fibrosis.
It is a key participant in generation of reactive oxidants and diffusible radical species by the phagocytes.
It serves as a potent toxin towards invading parasites and the surrounding tissues.
It also displays significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: 1:50 detected Eosinophil Peroxidase in frozen human colon tissue sections
Immunohistochemistry: formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Eosinophil Peroxidase.
Clone # USB：AHE-1
Isotype：IgG1,k
Calc Applications Abbrev：IC IF IHC
Calc Crossreactivity：Hu
Immunogen：Human Eosinophils from a patient with hypereosinophilic syndrome.
