83-5985-69　Pro-Atrial Natriuretic Peptide 100ug　052609

特徴

• Natriuretic peptides A (UniProt P01160; also known as Atriopeptin, Cardiodilatin, Cardiodilatin-related peptide, Cardionatrin, CDD, CDD-ANF, CDP, Natriuretic peptide precursor A, Prepronatriodilatin) is encoded by the NPPA (also known as ANF, ANP, ATFB6, ATRST2, PND) gene (Gene ID 4878) in human.
• Atrial natriuretic peptide (ANP), also called atrial natriuretic factor, is a cardiac hormone that reduces high blood pressure by promoting salt excretion, decreasing blood volume, and relaxing vessel tension in a receptor-dependent manner.
• Deficiency in either ANP or its receptor causes spontaneous hypertension in knockout mice.
• ANP is initially synthesized in cardiac myocytes as a 153 a.a.
• precursor with a signal (a.a.1-25) and a propeptide (a.a.
• 26-123) sequence.
• The removal of the signal peptide yields pro-ANP (a.a.
• 26-151), which is further cleaved by the transmembrane cardiac serine protease Corin to yield mature ANP (a.a.
• 124-151), also known as atrial natriuretic factor (ANF).
• ANP is a short-lived molecule partly due to its proteolytic degradation by the kidney Neprilysin, also known as neutral endopeptidase 24.11.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (FFPE): 1:1000 detected human Pro-Atrial Natriuretic Peptide in rat cardiac myocytes using heat-induced epitope retrieval (HIER)
• Western Blot: 1ug/mL detected human Pro-Atrial Natriuretic Peptide in 10ug of human heart tissue lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.