Phosphoprotein (UniProt: P69480; also known as Protein P, Protein M1) is encoded by the P gene in Rabies virus.
Rabies virus belongs to a family of RNA viruses called Rhabdoviridae, genus Lyssavirus.
It is most commonly transmitted to humans through the saliva of an infected animal.
This genus of virus shares many of the biological and physicochemical features that are associated with other rhabdoviruses.
They have similar mechanisms to enter susceptible cells, express and replicate their genome RNA and release mature virus particles from the plasma membrane of infected cells.
Rabies virus prototype is organized into five coding regions, N, P, M, G, and L.
Viral transcription and replication are catalyzed by the viral polymerase complex, composed of the L and P proteins.
Rabies virus is reported to bind to nicotinic acetylcholine receptors at the neuromuscular junction and travels toward the spinal cord within axons of peripheral nerves by retrograde fast axonal transport.
The virus disseminates within axons in the CNS along neuroanatomical pathways.
It replicates in neurons and causes neuronal dysfunction.
The phosphoprotein of Rabies virus is a non-catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication.
It is a multifunctional protein that is indispensable not only for viral replication but also for evasion of host innate immunity.
It establishes a trimeric form when phosphorylated.
It is reported to be phosphorylated at serine 162, 210, and 271 by protein kinase C.
The trimmer is stabilized by binding to the L protein.
It also binds to the soluble protein N+D12, preventing it from encapsidating non-genomic RNA.
It is reported to interact with host DYNLL1 and DYNLL2 via amino acids 138-172 and this interaction may play a role in intracellular microtubule-dependent virus transport of incoming virus.
It is also reported to inhibits host IFN- and IFN-β signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus.
Five isoforms of Rabies virus phosphoprotein have been described that are produced by alternative initiation.
052608 binds to the antigenic site consisting of amino acids 37-53 in the native P protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Inhibition Assays.
