CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin).
Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide.
Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical.
CD65 is expressed on granulocytes and monocytes and participates in cell adhesion.
It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 4ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
