HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin.
They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defence.
Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested
Recommended Dilution:Flow Cytometry: 10ul labels 10e6 cells in 100ul or 100ul whole bloodOptimal dilution determined by the researcher.
Excitation Laser:Blue (488nm)
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. Species Crossreactivity: bovine and non-human primates. Does not cross-block the antibody H6098-72R.
