052594 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide.
For use in ELISA or flow Cytometry, sample must first be reacted with NEM.
Applications:Suitable for use in Western Blot, ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry.
Immunocytochemistry: 1:100-1:500 for assessment of intracellular GSH distribution
ELISA: 1:100,000-1:200,000 to quantify GSH at >2mM in biological specimens
Flow Cytometry: 1:100-1:1000.
Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20℃) containing 5mM NEM (10 min on dry ice) or 10uM NEM (N-ethyl maleimide) for 30 min at RT
Optimal dilutions to be determined by the researcher.
Positive Control:Peripheral blood mononuclear cells (PBMC) treated with NEM
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Specificity：Recognizes thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins.
Clone # USB：8.1GSH
Isotype：IgG1
Calc Applications Abbrev：E FC IC IHC WB
Immunogen：GS-NEM conjugated to KLH. Species Sequence Homology: mammals
