The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag.
This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
052593 can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:5000Immunoprecipitation: 1:1000-1:1500Immunohistochemistry: 1:100-1:300Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes Myc-Tag.
Clone # USB：5F92F9
Isotype：IgG1
Calc Applications Abbrev：E IF IP WB
Immunogen：Synthetic peptide corresponding to Myc-Tag, conjugated to KLH
