83-5985-52　LIG1 (DNA Ligase 1) 100ul　052590

特徴

• LIG1 is the main replicative ligase involved in DNA replication, recombination, and the base excision repair process.
• LIG1 is associated with the replisome to finalize Okazaki fragment maturation, completing more than 50 million ligation events during each cycle of DNA replication.
• It also ligates single-strand DNA breaks during long-patch base excision repair (BER) and can participate in the alternative end-joining (A-EJ) pathway.
• LIG1 employs a unique metal-mediated fidelity mechanism whereby rigidity imposed by metal binding allows for discrimination against base mismatches on the 3′-hydroxyl side of the nick.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:5000
• Immunohistochemistry (paraffin): 1:50-1:200.
• Requires HIER
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.