83-5985-42　Nucleoporin p62, NT (NUP62) 100ul　052578

特徴

• Active transport of proteins and RNA into and out of the nucleus occurs via the nuclear pore complex (NPC).
• The NPC is formed by a multiprotein complex that includes nucleoporin proteins.
• Specific nuclear localization sequences found in proteins target proteins for active transport into the nucleus through the NPC.
• Nucleoporin p62 is the best characterized member of the family of nucleoporins found in the NPC.
• A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58.
• p54 binds to a carboxy-terminal coiled-coil domain of p62 and p58 binds to a dimer of p54.
• The amino-terminal domain of p62 contains a series of XFXFX repeats and is joined to the coiled-coil domain by a threonine-rich linker segment.
• The major role of p62 is maintenance of the structural integrity of NPCs.
• Applications:Suitable for use in Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500
• Immunocytochemistry: 1:50, specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.