83-5985-33　Mucin 1 (MUC1, Epithelial Membrane Antigen, EMA) 100ug　052571

特徴

• MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages.
• It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells.
• It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90% breast carcinomas and metastases.
• Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands.
• The MUC1 gene contains seven exons and produces several different alternatively spliced variants.
• The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain.
• The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
• Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT.
• Requires HIER
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.