Heat Shock Factor 1 (HSF1) is a key transcriptional regulator that orchestrates the cellular response to proteotoxic stress by activating genes involved in protein folding, degradation, and quality control.
As the primary mediator of the heat shock response, HSF1 binds to heat shock elements (HSEs) in the promoters of heat shock protein (HSP) genes, driving the expression of molecular chaperones essential for maintaining proteostasis.
Structurally, HSF1 contains a conserved N-terminal DNA-binding domain and adjacent hydrophobic repeats critical for trimerization and activation.
A second hydrophobic region near the C-terminus suppresses trimer formation under non-stress conditions.
In unstressed cells, HSF1 is diffusely localized in the cytoplasm and nucleus.
Upon exposure to stressors—such as oxidative damage, heat, or protein misfolding—HSF1 rapidly translocates to nuclear stress granules, initiating a transcriptional program that restores protein homeostasis.
In the context of neurodegenerative diseases, HSF1 plays a pivotal role in mitigating the toxic accumulation of misfolded proteins, a hallmark of disorders such as Alzheimer’s, Parkinson’s, and Huntington’s disease.
By enhancing the expression of protective HSPs, HSF1 supports neuronal survival and function under chronic stress conditions.
Its dynamic regulation and neuroprotective potential make HSF1 a compelling therapeutic target in neuroscience and neurodegeneration research.
Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:2000Immunocytochemistry/Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes mouse HSF1 at ~85kD (unstressed cell lysates), ~95kD (heat shocked cell lysates). Species Crossreactivity: human, monkey, rat, bovine, guinea pig, hamster and rabbit.
Clone # USB：4B4
Isotype：IgG1
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Bo Gp Hm Hu Mk Mo Rb Rt
Immunogen：Recombinant protein corresponding to mouse HSF1, epitope mapping to amino acids 425-439.
