Ubiquitin is a highly-conserved small protein expressed in all eukaryotes.
It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more than one ubiquitin monomers.
Ubiqutination controls the protein degradation, stability and intracellular localization of many proteins.
Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.125-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. The ubiquitin protein is extremLey well conserved and thus has extensive species cross-reactivity from yeast to human.
