Complement component C3 plays a key role in the activation of complement system.
In classical complement system pathway the activated C2 and C4 form classical C3-convertase (C4b2b) which cleaves C3 into C3a and C3b.
In alternative complement system pathway C3 is cleaved by alternative C3-convertase (C3bBb), composed of C3b (which can be generated also by spontaneous hydrolysis of C3) and the activated form of factor B.
C3b activates downstream cascade leading to formation of pores in the plasma membrane of attacked cell.
C3b and its proteolytically inactive form iC3b also serve as important opsonins.
C3b can generate C3f, as well as the iC3b can be further cleaved into C3c, C3d and C3g.
Complement system is regulated by effective inactivation of free C3b by factor H and factor I.
C3b attached to the surface of its target is protected from this inactivation.
Complement system is also regulated by other proteins, e.g.
CD35, CD46, CD55, or CD59.
Undesired activation of complement cascade can lead to severe diseases such as PNH, rheumatoid arthritis, macular degeneration and other.
Recently it has been demonstrated to take part in complications associated with Covid-19.
Applications:Suitable for use in Flow Cytometry Though not tested, has been reported to work in Immunocytochemistry, RIA and ELISA..
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 0.5-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes human C3b and iC3b. Does not cross-react with C3d. Species Crossreactivity: monkey
