83-5985-01　Active Caspase-3 100ug　052542

特徴

• Active caspase-3 is a protein encoded by the CASP3 gene which is approximately 31,6kD.
• Active caspase-3 is localised to the cytoplasm.
• It is involved in the TNFR1 pathway, apoptosis modulation and signalling and respiratory electron transport.
• This protein falls under the cysteine-aspartic acid protease family.
• It is a sequential activator of caspases and plays a central role in the execution-phase of cell apoptosis.
• Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.
• Active caspase-3 is highly expressed in lung, spleen, heart, liver and kidney.
• Mutations in the CASP3 gene may result in bladder urothelial carcinoma and primary effusion lymphoma.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:5000Immunohistochemistry (Paraffin): 1:500-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.