Fbrinogen is a 340kD glycoprotein secreted by the liver which circulates in plasma as a soluble homodimer.
The identical monomer subunits are linked by 17 disulfide bond and each subunit is composed of 3 polypeptide chains referred to as alpha, beta and gamma.
In response to bleeding, the coagulation cascade is activated which results in the conversion of fibrinogen to fibrin.
The resulting fibrin spontaneously forms polymers creating an insoluble gel which combines with platelets to form a spongy mass.
This mass is the blood clot which subsequently hardens to prevent further bleeding from the blood vessel (Tiscia & Margaglione 2018).
The impairment of this conversion from fibrinogen to fibrin is associated with a range of pathologies including coagulopathies, ischemic stroke and obstetrical complications.
It is also implicated in various neurological diseases such as Alzheimer’s, multiple sclerosis and traumatic central nervous system injury (Petersen et al.
2018).
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1ug/ml.
Can be used as a detection antibody with 052529 as capture antibodyOptimal dilutions to be determined by the researcher.
Hybridoma:Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
