GPV (platelet glycoprotein V; designated CD42d) is an 83kD type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family.
It is expressed exclusively within the platelet/megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIba/b and GPIX, at a rati o of one GPV to two of each other subunit.
The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells.
Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder.
The human GPV cDNA encodes a 560aa protein with a 16aa signal sequence, a 507aa extracellular domain (ECD) containing 15 LRR, a 21aa TM sequence, and a short (16aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets.
The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively.
GPV can form soluble fragments of 80kD by ADAM10 or ADAM17 cleavage after P507, or 69kD by thrombin cleavage after R476.
High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin.
The function of GPV is not entirely clear.
Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb.
Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul to label 10e6 cells with human peripheral blood plateletsOptimal dilutions to be determined by the researcher.
Absorption:620-650nm
Emission:660-670nm
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Glycoprotein V. Does not crossreact with recombinant human Glycoprotein VI.
Clone # USB：25G5
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln17-Gly523 of human Glycoprotein V, expressed in NS0 cells. Species Sequence Homology: equine; 81%, rat; 71%, mouse; 70%.
