ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family.
ROR gamma is a DNA binding transcription factor.
ROR gamma is 518 amino acids (aa) in length.
Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis.
Two splice forms differing in the first 24aa have been found for this gene; isoform 2 deletes aa1‑21 resulting in an alteration of aa22‑24.
Over aa1‑121 human ROR gamma shares 97% identity with mouse ROR gamma.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml with Daudi human Burkitt's lymphoma cell line, SUP-T1 human T cell lymphoblastic lymphoma cell line and Jurkat human acute T cell leukemia cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes recombinant human ROR gamma T, RORA and RORB.
Clone # USB：25G1
Isotype：IgG2b
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala100 from human RORg, expressed in E. coli.
