83-5984-67　Chlamydia trachomatis LPS 100ug　052517

特徴

• Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV).
• Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants.
• These are identified based on serological reactivity of the epitopes on their outer membrane.
• Intracellularly chlamydia replicates within a vacuole.
• Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles.
• This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell.
• Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate.
• Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS.
• It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water.
• Applications:Suitable for use in Electron Microscopy, Immunofluorescence, Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:
• Western Blot: 2ug/ml detected Chlamydial LPS in HeLa cells infected with chlamydia trachomatis serovar L2 (LGV 434 L2)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.