Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1).
This antibody can be used in flow cytometry or various assay formats to measure Her2 expression.
Applications:Suitable for use in Flow Cytometry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul labels 1x10e6 cells in MCF-7 human cell lineImmunocytochemistry: 5-25ug/ml with live and fixed SK-BR-3 human breast cancer cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：human
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes human ErbB2/Her2 This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab.
