Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Neutralization Assay, Affinity chromatography, Dot Blot and ELISA.
Not suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence (IC): 1:20Dot Blot: 1:500 (0.1ug/ml; non-denaturing conditions)Immunoprecipitation: 1:5Neutralization: EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃.
For long-term storage, aliquot and store at 4℃.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mL
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide.
Specificity：Recognizes AAV-2. Reacts with AAV2, AAV2.7m8 and AAV3. Specifically reacts wtih intact AAV particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids not present in denatured capsid proteins and native but unassembled capsid proteins. Major reaction attributed to aa369-378 of AAV-2 capsids. Does not react with AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10 or AAVrh74.
