83-5984-42　CD14 (PE) 100Tests　052495

特徴

• Custered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al.
• 2001).
• Immunoprecipitates a protein of ~50kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies.
• Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thacker et al.
• 2001).
• Clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry.
• Lymphocytes and eosinophils are negative for MIL2 staining (Zelnickova et al.
• 2007).
• Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells.
• (Petersen et al.2007 and Sanz et al.
• 2007).
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: Neat.
• 1:2, 10ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
• Hybridoma:P2-X63-Ag.653 myeloma cells with spleen cells from Balb/c mice.
• Storage and Stability:Store product at 4℃ before and after reconstitution.
• DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4℃.
• Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product centrifuge the original vial prior to removing the cap.