83-5984-36　EGFRvIII, Mutant 100ug　052479

特徴

• Epidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human.
• EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed.
• It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved.
• It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210).
• Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades.
• EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes.
• The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain.
• EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC).
• It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance.
• Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth.
• Applications:Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 4ug/ml detected EGFRvIII in 10ug of U87MG.delta EGFR cell lysates
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.