Epidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human.
EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed.
It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved.
It contains an extracellular domain (aa25-645) a transmembrane domain (aa646-668), and a cytoplasmic domain (aa669-1210).
Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades.
EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes.
The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain.
EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC).
It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance.
Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth.
052478 binds specifically to the epidermal growth factor receptor variant III (EGFRvIII), which is present on gliomas, but not normal tissues, and is internalized rapidly after receptor binding.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml detected EGFRvIII mutation in NR6 cells expressing human EGFRvIII (NR6M)
Immunohistochemistry (Paraffin): Requires HIER using sodium citrate buffer pH 6.0 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes EGFRvIII, a mutated form of EGFR.
Clone # USB：L8A4
Isotype：IgG1,k
Calc Applications Abbrev：E FC IC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to the predicted amino acid sequence of 14 amino acids at the fusion junction combined with cultured HC2 20 d2 cells.
