Siglec-1, also known as sialoadhesin or CD169, is a 175‑185kD type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily.
Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains.
The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively.
Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains.
Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections.
It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 cells using RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight Optimal dilutions to be determined by the researcher.
Excitation:488nm
Emission:565-605nm
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromotography from hybridoma culture supernatant
Form：Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes mouse Siglec-1. Does not react with recombinant human Siglec-1, 5, 6, 7, 8, 9, 10, 11 or -14. No crossreactivity is observed with recombinant mouse Siglec-2, -3, -E, -F, -G, or -H.
Clone # USB：25E1
Isotype：IgG2a
Calc Applications Abbrev：FC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Thr20-Leu1639 (predicted) of mouse Siglec-1, expressed in NS0 cells
