83-5984-33　Siglec 1 (Sialic Acid Binding Ig-like Lectin 1, Sialoadhesin, CD169) 100ug　052475

特徴

• Siglec-1, also known as sialoadhesin or CD169, is a 175‑185kD type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily.
• Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains.
• The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively.
• Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains.
• Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections.
• It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
• Applications:Suitable for use in Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml using lysates of mouse splenocytesFlow Cytometry: 0.25ug/10e6 cells with RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight Optimal dilutions to be determined by the researcher.
• Storage and Stablility:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.