Glutamic acid decarboxylase 65 (GAD65), also known as GAD2, catalyzes the production of GABA from L-glutamic acid and is encoded by GAD2 in humans.
It is primarily expressed in pancreatic islets but is also expressed in neurons of the central and peripheral nervous systems and is localized to vesicular membranes.
Autoantibodies against GAD65 have been found in patients with type 1 diabetes mellitus or gestational diabetes mellitus and have been used as biomarkers of type 1 diabetes mellitus.
GAD65 autoantibodies have also been found in patients with the rare neurological disorder stiff-man syndrome or autoimmune epilepsy
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000ELISA: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GAD65. Species Crossreactivity: mouse
Clone # USB：9B10
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Full-length recombinant protein corresponding to human GAD65
