The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner (1).
Different members of the family were discovered by different laboratories and as a result the various members have many alternate names, such as lipocortin, calpactin, calelectrin and others.
In fact Annexin A5 has a particularly surprising number of alternate names, 20 being listed on the Genecards ANXA5 site.
The widely used current nomenclature is now based on a letter to indicate membership in a particular one of several annexin sub-families and a number for individual gene products, hence the name annexin A5.
The annexin family is defined by a compact disc structure formed from 16 closely packed α-helices which coordinate multiple calcium ions with phospholipid containing membranes.
This domain is defined by 4 imperfect repeats of a ~77aa sequence, each repeat forming 4 α-helices (2,3).
Annexin A5 is expressed widely in tissues and has been used as a marker of apoptosis, as apoptotic cells may express binding sites for this protein on their cell surface.
The protein binds to phosphatidylserine, a membrane lipid normally not found on the external surface of cells which becomes expressed on the cell surface during apoptosis.
As a result fluorescent annexin A5 or annexin A5 antibody can be used to isolate apoptotic cells by fluorescence activated cell sorting (4).
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000-1:5000Immunohistochemistry: 1:1000-1:2000Immunofluorescence (IC): 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Specificity：Recognizes human annexin A5. Does not recognize annexin A5 in rodents and many other mammals. Does recognize annexin A5 in COS1 cells which originated from African green monkey.
Clone # USB：6A12
Isotype：IgG1
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Full length recombinant protein corresponding to human annexin A5 expressed in E. coli.
