CD16, a 50-65kD cell surface molecule, which is the low affinity receptor for IgG (FcR III).
CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B).
CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al.
1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al.
1999).
Recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Hybridoma:P3U1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:Store product at 4℃ before and after reconstitution.
DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4℃.
Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from tissue culture supernatant.
Form：Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1mL sterile ddH2O.
Specificity：Recognizes human CD16. Species Crossreactivity: bovine, sheep, dolphin. Reacts weakly with equine and porcine. Does not react with canine and rat.
