83-5984-24　NR5A2 100ul　052457

特徴

• Liver receptor homolog 1 (LRH-1, CPF, FTF, PHR-1, FTF, or hB1F) is an orphan nuclear receptor that is highly expressed in the intestines, pancreas, ovary, and liver.
• LRH-1 is a constitutively active transcription factor with a complex role in many cellular processes which may include lipid metabolism, cell proliferation, development, reproduction, and immunity.
• LRH-1 may interact with an array of transcriptional coactivators that modulate its activity, among which are the MBF1, SRC, and PGC-1α proteins.
• In addition, ERK phosphorylation on Ser-238 and Ser-243 also enhances LRH-1 activity.
• However, LRH-1 is also negatively regulated by several factors.
• For instance, corepressors such as SHP, DAX-1, and PROX-1, preclude its binding to DNA; and sumoylation promotes its inactivation and sequestration into nuclear bodies.
• LRH-1 targets many genes including the CYP7A1 and CYP8B1 genes involved in bile synthesis; and Oct4 required for pluripotency in embryonic stem cells.
• LRH-1 has been implicated in inflammatory bowel disease, and tumor development in the intestines and pancreas.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 detected Liver receptor homolog-1 in 10ug of mouse pancrease tissue lysate
• Optimal dilutions to be determined by the researcher.
• Control:Mouse pancreas tissue lysate
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.