Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation.
The mature myeloperoxidase is a tetramer composed of two light (12kD) and two heavy (60kD) chains.
This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens.
Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 4ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal working dilutions to be determined by researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human myeloperoxidase (MPO).
