83-5984-14　CXCR7 (C-X-C Chemokine Receptor 7, Chemokine Orphan Receptor 1, RDC-1) 100ug　052448

特徴

• The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7.
• CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC.
• It is also a co-receptor for several HIV and SIV strains.
• In their N-termini and extracellular loops 1, 2 and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively.
• Reports of mRNA levels and/or protein expression indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells.
• In contrast, based on ligand binding analysis and receptor level (assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells and few other cell types.
• The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.
• Applications:Suitable for use in Immunocytochemistry and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunocytochemistry: 8-25ug/ml with immersion fixed MCF‑7 human breast cancer cell lineImmunohistochemistry (Paraffin): 0.5-25ug/ml.
• Detected CXCR7 in immersion fixed paraffin-embedded sections of human breast cancer tissueOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.