G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage.
It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma.
Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation.
In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein.
Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage.
It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors.
Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml using recombinant Mouse G-CSFELISA (capture): 2-8ug/ml using 052446 as detection reagentNeutralization: Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line.
Shirafuji, N.
et al.
(1989) Exp.
Hematol.
17:116.
The Neutralization Dose (ND50) is typically 0.01-0.03ug/mL in the presence of 0.125ng/m recombinant mouse G‑CSF
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from culture supernatant.. Endotoxin: ≤0.1EU/ug (LAL).
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes mouse G-CSF. Does not react with with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
Clone # USB：25C15
Isotype：IgG1
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val31-Ala208 from mouse G-CSF expressed in E. coli.
