The FTO gene is the most robust gene for common obesity characterized to date.
FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008).
Deletion analysis of FTO gene in mice showed that FTO is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunocytochemistry: 1:100-1:600Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from culture supernatant..
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of FTO. Species Crossreactivity: human, rat and mouse
Clone # USB：5-2H10
Isotype：IgG2a
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat FTO, conjugated to KLH. Species Sequence Homology: bovine, canine, primate and sheep
