Major prion protein (UniProt: P04925; also known as PrP, PrP27-30, PrP33-35C, CD230) is encoded by the Prnp (also known as Prn-p, Prp) gene (Gene ID: 19122) in murine species.
PrP is highly expressed in the brain, lung, kidney and heart and low levels of expression are reported in the liver and spleen.
It is found in high quantity in the brain of humans and animals infected with degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), scrapie, bovine spongiform encephalopathy (BSE), and transmissible mink encephalopathy (TME).
In normal brain it may play a role in neuronal development and synaptic plasticity and may be required for neuronal myelin sheath maintenance and myelin homeostasis through acting as an agonist for ADGRG6 receptor.
PrP can exist in either PrPc (c = cellular) or PrPsc (sc = Scrapie) form.
PrPc is a glycoprotein normally found inserted in the plasma membrane by a glycosyl phosphatidylinositol (GPI) anchor and can be easily digested by proteases.
It is composed mainly of alpha-helices.
The PrPsc has more of a beta-sheet type structure and is highly resistant to the action of proteases.
The prion proteins possess two N-linked glycosylation sites and may contain over 50 different sugars.
The large size of the N-linked sugars enables them to shield two orthogonal faces of the protein almost completely and protect large regions of the protein surface from proteases.
The PrPsc is shown to contain the same set of glycans as PrPc, but has a higher proportion of tri- and tetra-antennary sugars.
Multiplication of prion proteins appears to be slower in vivo than in vitro.
It is shown that following proteinase K treatment, PrPc is completely digested, whereas PrPsc is only shortened to a 27-30kD fragment.
Applications:Suitable for use in ELISA, Immunohistochemistry, Western Blot, Immunofluorescence and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5ug/ml detected PrP in Brain homogenate from a Tga20 mouse overexpressing Prnp versus brain homogenate from a ZH3/ZH3 control mouse lacking Prnp
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes major prion protein in Tga20 mice brain. Targets an epitope within C-terminal globular domain. Species Crossreactivity: human
Clone # USB：POM1
Isotype：IgG1,k
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：GST-tagged recombinant fragment corresponding to 209 amino acids from the internal region of mouse major prion protein.
