LL-37 is a cationic α-helical peptide expressed in human bone marrow, testis, granulocytes, and gingival epithelium and a variety of immune cells.
It has antimicrobial and antiviral activity, and protein levels of LL-37 are increased in epithelial cells, macrophages, and neutrophils following bacterial infection in vitro.
LL-37 can be citrullinated by protein arginine deiminase 2 (PAD2) and PAD4, a modification that reduces its antibacterial and antiviral activities.
Citrullinated, unlike native, LL-37 does not prevent mortality in a mouse model of D-galactosamine-sensitized endotoxic shock.
Citrullinated LL-37 has been found in neutrophil extracellular traps (NETs), as well as the serum of patients with rheumatoid arthritis (RA) and individuals without RA.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000ELISA: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：300ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes citrullinated LL-37 at ~9kD from human samples. Does not react with Native LL-37.
