83-5983-66　Keratan Sulfate 100ug　052373

特徴

• Keratan sulfate (also known as keratosulfate) is a widely distributed glycosaminoglycan in tensional and weight-bearing connective tissues, epithelial tissues, and the central and peripheral nervous system.
• It is attached to a number of proteoglycan and glycoprotein core proteins.
• Three types of keratan sulfate have been described: KS I, KS II, and KS III depending on their distribution pattern.
• Keratan sulfate is the only glycosaminoglycan (GAG) that does not contain uronic acid; therefore, it is not cleavable by the eliminative enzymatic cleavage.
• The biosynthesis of keratan sulfate occurs in two stages.
• In the first stage, the region binding the core protein with GAG is created followed by the elongation of the chain and its modification.
• The elongation of keratan sulfate chains of all types occurs by alternate attaching of Gal and GlcNAc residues, catalyzed by β-1,4-galactotransferase and β-1,3-N-acetyloglucosaminotransferase, respectively.
• Sulfation of keratan sulfate is carried out by at least two sulfotransferases.
• Gal 6-O-sulfotransferase transfers sulfate to position 6 of Gal residues located in the internal region of keratan sulfate and GlcNAc 6-O-sulfotransferase transfers sulfate to position 6 of GalNAc residues located at the non-reducing terminal of keratan sulfate.
• Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (paraffin): 1:50 detected Keratan Sulfate in human cartilage tissue sections using heat-induced epitope retrieval (HIER)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.