R2032-24D has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated.
It is possible that complement deposition on rituximab opsonised cells inhibits binding of the Anti-Rituximab Antibody to cell bound rituximab (Beum et al.
2004).
Inhibition experiments carried out with Daudi cells demonstrated that this antibody is inhibitory at a ratio of 5:1 antibody:rituximab, but does not inhibit rituximab binding to CD20 at a ratio of 1:1.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis.
The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells.
Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:200,000Optimal dilutions to be determined by the researcher.
Hybridoma:Spleen cells from immunized rats were fused with cells of the NS-1 myeloma cell line.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, 0.01% Thiomersal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes the idiotypic determinants expressed by the Rituximab humanized monoclonal antibody. Does not recognize other CD20 antibodies. Also recognizes ocrelizumab, a second-generation humanized anti-CD20 antibody that binds to an epitope on CD20 that is identical or overlapping with the rituximab epitope.
