The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases.
This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps.
Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner.
Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-4ug/ml.
Intracellular staining
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Western Blot Positive Control:EGF-stimulated A431
Western Blot Negative Control:Nnon-stimulated A431.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human EGFR phosphorylated on intracellular tyrosine 992.
Clone # USB：EM-12
Isotype：IgG1
Calc Applications Abbrev：FC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phospho-peptide corresponding to the sequence around tyrosine 992 of human EGFR.
